4T1 Breast Cancer mouse model: A Reliable Tool for Breast Cancer Research

4T1 is carcinoma cell line originating from a spontaneous mammary tumor in a Balb/c mouse commonly used in preclinical studies of breast cancer. This cell line was derived from a spontaneous mammary tumor in a Balb/c mouse and been extensively researched due to its capacity to generate highly metastatic tumors, closely mirroring the characteristics of human breast cancer in murine models.

Target:

Cancer

Date:

April 11, 2024

Category:

The major advantage of this model is the orthotopic implantation of the 4T1 breast cancer cells in the breast fat pad, offering a proper tissue microenvironment that is known to affect tumor properties and drug resistance, increasing the clinical relevance and predictivity over traditionally used subcutaneous models that lack the proper microenvironment.

Model Description

A small volume of the tumor cell suspension is injected into the mammary fat pads of anesthetized female Balb-c  mice. Mice are monitored daily and weight is recorded three times per week.

Tumors are expected to become palpable in about 5 days following injection, and from that point on are measured by calipers to calculate tumor volume.

Arthritic Score and Body Weight

Tg197 mice exhibit progressive worsening arthritis and reduced body weight gain due to the human TNF overexpression that causes cachexia

Circulating Cytokine Levels

Tg197 mice exhibit increased levels of serum circulating human TNF and mouse IL-6

Preclinical Platform

In vivo: Treatments can start from day 3 following tumor inoculation and can be administered systemically or locally. Study duration is up to three weeks or until the tumors reach 1500mm3  of tumor volume.

In vitro: 4T1 cell line is also available for in vitro cellular assays, such as cell apoptosis, proliferation, viability and would healing

Enhance Your Research Strategy Today

Partner with Our Skilled Scientists and discover Cutting-Edge Solutions!
Contact Us